Sensory Evaluation and Shelflife of Smoke-Cured Clarias Gariepinus Preserved with Different Sources of Fuel Energy

Authors

  • J. E. Obugara Delta State University Abraka Author
  • A. A. Nwabueze Delta State University Abraka Author
  • P. U. Okoro Federal Polytechnic Orogun Author
  • P. N. Okpu Southern Delta University Ozoro Author

DOI:

https://doi.org/10.70882/josrar.2026.v3i1.139

Keywords:

Microbial, Shelf life, Proximate, Sensory qualities, Lipid oxidation

Abstract

The impact of three smoking media (firewood, charcoal, and sawdust) on the sensory qualities and nutritional composition, lipid oxidation products, and microbial quality of smoked Clarias gariepinus during 28 days storage at room temperature was investigated in this study. Fish samples were tested on Days 0, 7, 14, 21 and at day 28. According to the sensory analysis of results, at Day 0 all samples were very well accepted were taste (8.03-8.58), aroma (8.23–8.45), texture (8.43–9) and color (7.83­-9). However, scores decreased over time (p < 0.05) to 1.09–1.82 on Day 28. The firewood-smoked fish had the best sensory scores throughout storage (taste: 5.00). Proximate composition indicated a gradual decline in crude protein from Day 0 to Day 28. Firewood-smoked fish decreased from 44.71% to 19.92%, charcoal from 39.42% to 16.03%, and sawdust from 37.54% to 10.02%. Lipid oxidation (TBARS) increased steadily, with sawdust attaining the highest value (10.74 mg MDA/kg) at Day 28, compared to charcoal (10.56 mg MDA/kg) and firewood (10.10 mg MDA/kg). Microbial analysis showed substantial bacterial and fungal loads from the start, ranging between 2.8×10⁶–9.1×10⁶ CFU/ml for bacteria and 1.7×10⁶–6.1×10⁶ CFU/ml for fungi on Day 0. Microbial counts rose consistently during storage, with sawdust-smoked samples recording the highest loads (>10⁷ CFU/ml by Day 28), while firewood-treated samples showed comparatively lower microbial growth. Overall, firewood emerged as the most effective smoking medium, producing fish with superior sensory quality, higher protein retention, lower lipid oxidation, and better microbial stability. The study demonstrates that smoking medium significantly influences both the shelf life and safety of smoked catfish, underscoring the need for improved smoking technologies and post-processing hygiene to minimize spoilage and enhance product quality.

References

Abdel-Naeem, H. H., Sallam, K. I., and Malak, N. M. (2021). Improvement of the microbial quality, antioxidant activity, phenolic and flavonoid contents, and shelf life of smoked herring (Clupea harengus) during frozen storage by using chitosan edible coating. Food Control, 130, 108317.

Abdul, I. M., Kamilu, M., Maiyanga, I. E., Yaji, I. L., Yerima, A. K and Ibrahim, A. T. (2024). The Role of Post-Harvest Technologies in the Handling and Preservation of Fish and Fish Products in Nigeria: A Mini-Review. Journal of Agriculture, Aquaculture, and Animal Science, 1(1), 1-4.

Adeyeye, S. A. O. (2019). Smoking of fish: a critical review. Journal of Culinary Science & Technology, 17(6), 559-575.

Adeyeye, S. A. O., Oyewole, O. B., Obadina, O., Adeniran, O. E., Oyedele, H. A., Olugbile, A., and Omemu, A. M. (2016). Effect of smoking methods on microbial safety, polycyclic aromatic hydrocarbon, and heavy metal concentrations of traditional smoked fish from Lagos State, Nigeria. Journal of Culinary Science & Technology, 14(2), 91-106.

Agbangba, C. E., Aide, E. S., Honfo, H., and Kakai, R. G. (2024). On the use of post-hoc tests in environmental and biological sciences: A critical review. Heliyon, 10(3).

Ahmed, N., Thompson, S., and Glaser, M. (2019). Global aquaculture productivity, environmental sustainability, and climate change adaptability. Environmental management, 63, 159-172.

Akinwole, A. O., Fawole, O. O., and Oyediran, F. S. (2024). Nutritive and organoleptic evaluation of the African catfish (Clarias gariepinus) smoked with briquette and charcoal. Journal of Agriculture and Food Sciences, 22(1), 69–76.

Alosias, J. (2020). A review on the application of improved fish handling and smoking: safe quality and potential business venture for developing countries. London Journal of Research in Science: Natural and Formal.

Aneja, K.R. (2018). A Textbook of Basic and Applied Microbiology, New Age International Publishers, New Delhi.

Arvanitoyannis, I. S., and Kotsanopoulos, K. V. (2012). Smoking of fish and seafood: history, methods and effects on physical, nutritional and microbiological properties. Food and bioprocess technology, 5, 831-853.

Asamoah, E. (2018). The quality and shelf life of Atlantic mackerel (Scomber scombrus) as influenced by smoking technology, packaging material and storage temperature. Nations University Fisheries Training Programme, Iceland[Final Project].

Asen, B., Akombo, P. M., Annune, P. A and Nyakuma, B. B. (2024). Assessing Nutrient Retention, Cost-Effectiveness and Fuel Efficiency of Charcoal Briquettes for Fish Smoking in Nigeria. Engineering Proceedings, 67(1), 77.

Assogba, M. F., Anihouvi, E. L., Adinsi, L., Boukary, B. S., Kpoclou, Y. E., Mahillon, J., and Anihouvi, V. B. (2021). Sensory profiling of meat and fish products obtained by traditional grilling, smoking and smoking-drying processes. Journal of Aquatic Food Product Technology, 30(4), 378-391.

Awhefeada, O. K., Bekederemo, B. O., Akpomughe, E., & Okpu, P. N. (2025). Morphometric Characterization, Heterosis Expression, and Growth Performance of Genetically Improved Farmed Tilapia and Red Tilapia Hybrids for Sustainable Aquaculture Development. Journal of Science Research and Reviews, 2(2), 145-155. https://doi.org/10.70882/josrar.2025.v2i2.102

Badoni, P., Nazir, I., Aier, M., Maity, P. B., Samanta, S., and Das, A. (2021). Significant role of fish nutrients with special emphasis to essential fatty acid in human nutrition. Int. J. Curr. Microbiol. Appl. Sci, 10, 2034-2046.

Budaraga, I. K. (2017). Effect of Combination Treatment of Liquid Smoke Concentration, Soaking Time, Packaging and Different Storage Time To Yield And Moisture Content nila Fish Fillet (Oreochromis niloticus). International Journal of ChemTech Research, 10(1), 77-88.

Cheesbrough, M. (2002). District Laboratory Practice in Tropical Countries (Part 2). Trpical Health Technology Publishers, Greatn Britain 40-56pp.

Cyprian, O. O., Van Nguyen, M., Sveinsdottir, K., Jonsson, A., Tomasson, T., Thorkelsson, G., and Arason, S. (2015). Influence of smoking and packaging methods on lipid stability and microbial quality of C apelin (M allotus villosus) and Sardine (S ardinella gibossa). Food science & nutrition, 3(5), 404-414.

Daramola, J. A., Fasakin, E. A., and Adeparusi, E. O. (2007). Changes in physicochemical and sensory characteristics of smoke-dried fish species stored at ambient temperature. African Journal of Food, Agriculture, Nutrition and Development, 7(6).

Daramola, J. A., Fasakin, E. A., and Famurewa, J. A. V. (2020). Fish smoking kiln using agricultural wastes as energy source (A). The International Journal of Engineering and Science, 9(4), 29–33.

Don, S., Lekshmi, M., and Kumar, S. H. (2025). Microbiological quality and safety of processed and packaged fish and fishery products. In Quality Analysis and Packaging of Seafood Products (pp. 305-333). Academic Press.

Ekelemu, J. K. Nwabueze, A. A. Irabor, A. E. and Otuye, N. J. (2021). Spicing: A means of improving organoleptic quality and shelf life of smoked catfish. Scientific African, 13; 1-7. E00930. https://doi.org/10.1016/j.sciaf.2021.e00930.

Geraldo, V. (2023). Good Manufacturing Practices (Gmp) To Improve The Safety And Quality Of Smoked Fish (Doctoral dissertation, University of Cape Coast).

Huong, D. T. T. (2014). The effect of smoking methods on the quality of smoked mackerel. United Nations University Fisheries Training Programme, Iceland [final project].

Hussein, M. A., Merwad, A. M., Elabbasy, M. T., Suelam, I. I., Abdelwahab, A. M., and Taha, M. A. (2019). Prevalence of enterotoxigenic Staphylococcus aureus and Shiga toxin-producing Escherichia coli in fish in Egypt: Quality parameters and public health hazard. Vector-Borne and Zoonotic Diseases, 19(4), 255–264. https://doi.org/10.1089/vbz.2018.2336

Idah, P. A., and Nwankwo, I. (2013). Effects of smoke-drying temperatures and time on physical and microbial quality of fish. African Journal of Agricultural Research, 8(46), 5932–5937. https://doi.org/10.5897/AJAR2013.7222

Krishnan-Sarin, S., O’Malley, S. S., Green, B. G and Jordt, S. E. (2019). The science of flavour in tobacco products. World Health Organization technical report series, 1015, 125.

Langi, S., Maulu, S., Hasimuna, O. J., Kaleinasho Kapula, V., and Tjipute, M. (2024). Nutritional requirements and effect of culture conditions on the performance of the African catfish (Clarias gariepinus): a review. Cogent Food and Agriculture, 10(1), 2302642.

Lindgren, E., Harris, F., Dangour, A. D., Gasparatos, A., Hiramatsu, M., Javadi, F and Haines, A. (2018). Sustainable food systems—a health perspective. Sustainability science, 13, 1505-1517.

Majumdar, R. K. (2019). Traditional Technology of Fish Preservation in Northeast India. In Technologies for Value Addition in Food Products and Processes (pp. 127–162). Apple Academic Press.

March, A., and Failler, P. (2022). Small-scale fisheries development in Africa: Lessons learned and best practices for enhancing food security and livelihoods. Marine Policy, 136, 104925.

Messina, C. M., Arena, R., Ficano, G., Randazzo, M., Morghese, M., La Barbera, L and Santulli, A. (2021). Effect of cold smoking and natural antioxidants on quality traits, safety and shelf life of farmed meagre (Argyrosomus regius) fillets, as a strategy to diversify aquaculture products. Foods, 10(11), 2522.

Muringai, R. T., Mafongoya, P., Lottering, R. T., Mugandani, R., and Naidoo, D. (2021). Unlocking the potential of fish to improve food and nutrition security in sub-Saharan Africa. Sustainability, 14(1), 318.

Nwabueze, A. A. and Atokpe, I. Q. (2015). Fish smoking: A post-harvest technology in Delta State, Nigeria. Proceedings of the 49th Annual Conference of the Agricultural Society of Nigeria. Asaba 2015 9th-13th November, 2015.1202-1206pp.

Ojelade, O. C., George, F. O. A., Abdulraheem, I., and Akinde, A. O. (2023). Interactions between pre-harvest, post-harvest handling and welfare of fish for sustainability in the aquaculture sector. In Emerging Sustainable Aquaculture Innovations in Africa (pp. 525-541). Singapore: Springer Nature Singapore.

Okpala, C. O. R and Korzeniowska, M. (2023). Understanding the relevance of quality management in agro-food product industry: From ethical considerations to assuring food hygiene quality safety standards and its associated processes. Food Reviews International, 39(4), 1879-1952.

Oli, A. U., Okeke, P. A., Oli, C. C., Ibe, F. N., and Okogwu, J. D. (2024). Comparative Study of the Proximate Composition and Sensory Evaluation of Fresh and Smoke-dried Fish Species from Omambala River, Anambra State, Nigeria. APPLIED SCIENCES (NIJBAS), 5(2).

Osineye, O., Abiodun-Solanke, A. J., Mangai, E., Okeke, E., and Jahnezim, B. (2020). Comparison of polyaromatic hydrocarbon residue concentrations in Clarias gariepinus smoked with traditional and mechanical kilns. Journal of Health and Pollution, 10(28), 201215.

Ovie, K. A., Okpu, P., Oster, F. N., & Emuesiri, A. (2025). Evaluation of Proximate Composition of Varying Black Soldier Fly (BSF) Larvae as an Alternative Protein Source. Journal of Science Research and Reviews, 2(4), 1-8. https://doi.org/10.70882/josrar.2025.v2i4.95

Prajapat, B. (2024). Fish Fermentation: Techniques and Uses. Educohack Press.

Puke, S., and Galoburda, R. (2020). Factors affecting smoked fish quality: A review. Research for rural development, 35(March), 132-139.

Rana, M. M., Mohibbullah, M., Won, N. E., Baten, M. A., Sohn, J. H., Kim, J. S., and Choi, J. S. (2021). Improved hot smoke processing of chub mackerel (Scomber japonicus) promotes sensorial, physicochemical and microbiological characteristics. Applied Sciences, 11(6), 2629.

Soares, J. M., da Silva, P. F., Puton, B. M. S., Brustolin, A. P., Cansian, R. L., Dallago, R. M., and Valduga, E. (2016). Antimicrobial and antioxidant activity of liquid smoke and its potential application to bacon. Innovative Food Science & Emerging Technologies, 38, 189-197.

Taherzadeh-Shalmaei, N., Sharifi, M., Ghasemi-Mobtaker, H., and Kaab, A. (2021). Evaluating the energy use, economic and environmental sustainability for smoked fish production from life cycle assessment point of view (case study: Guilan Province, Iran). Environmental Science and Pollution Research, 28(38), 53833-53846.

Tigabu, Y., Yimer, A., Mikael, F. H and Tadesse, S. (2022). Suitability of Wooden Plants for Making Smoked Products from Nile Tilapia Fish. Livestock Research Results, 544.

Wijerathna-Yapa, A., and Pathirana, R. (2022). Sustainable agro-food systems for addressing climate change and food security. Agriculture, 12(10), 1554.

Yang, J and Lee, J. (2018). Korean consumers’ acceptability of commercial food products and usage of the 9‐point hedonic scale. Journal of Sensory Studies, 33(6), e12467.

Zaki, H. M. B. A., Emara, M. M. T and Abdallah, M. R. S. (2021). Effect of smoke duration on compositional analysis, deterioration criteria, microbial profile and sensory attributes of marine and freshwater fish: a comparative study. Adv. Anim. Vet. Sci, 9(8), 1259-1266.

Showing Moisture Content for Day 0,7,14,21 and 28

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Published

2026-02-25

How to Cite

Obugara, J. E., Nwabueze, A. A., Okoro, P. U., & Okpu, P. N. (2026). Sensory Evaluation and Shelflife of Smoke-Cured Clarias Gariepinus Preserved with Different Sources of Fuel Energy. Journal of Science Research and Reviews, 3(1), 114-132. https://doi.org/10.70882/josrar.2026.v3i1.139